Anthropogenic and naturally occurring polybrominated phenolic compounds in the blood of cetaceans stranded along Japanese coastal waters

We determined the residue levels and patterns of hydroxylated polybrominated diphenyl ethers (OH-PBDEs), and related compounds, such as PBDEs, methoxylated PBDEs (MeO-PBDEs), and bromophenols (BPhs) in the blood of eleven cetacean species stranded along the Japanese coasts. The dominant OH- and MeO-PBDE isomers found in all cetaceans were 6OH-BDE47 and 6MeO-BDE47. Additionally, 2,4,6-triBPh was dominant isomer in all cetaceans. In contrast, specific differences in the distribution of para- and meta- OH-PBDE isomers and some BPhs (potential PBDEs metabolites) were found among the cetaceans.Residue levels of ΣMeO-PBDEs and 6OH-BDE47 + 2′OH-BDE68, and 2,4,6-triBPh and 6OH-BDE47 + 2′OH-BDE68 showed a significant positive correlation. These results may suggest that the large percentages of OH-PBDEs, MeO-PBDEs and 2,4,6-triBPh might share common source (i.e. biosynthesis by marine organisms), or metabolic pathway in cetacean species. Significant correlations were found between the concentrations of BDE99 and 2,4,5-triBPh. This result suggested that 2,4,5-triBPh in cetaceans could be a metabolite of BDE99.     

Capability development of local communities for project sustainability in afforestation/reforestation clean development mechanism

It has been recognized that the involvement of local community is essential to ensure the sustainability of A/R CDM (afforestation/reforestation clean development mechanism) project. This study verifies if the risks of non-permanence and leakage are addressed in a registered small scale A/R CDM project in Vietnam. Workshops, interviews, and a questionnaire survey of local villagers revealed that the project has caused a shortage of land for conventional activities such as grazing, fuel wood collection and shifting cultivation, and consequently posed the risks of project non-permanence and leakage. It is suggested that participation of all stakeholders in the community to the A/R CDM project beyond existing land tenure and adequate carbon benefit sharing according to the level of contribution to the project are required to reduce the risk of non permanence. To ensure the participation, the community should have capability such as consensus building and collective action. Leakage would be minimized if the community has alternative measures to the conventional activities before starting the project. We argue that it is necessary to first develop a community’s capabilities in the readiness phase of any A/R CDM project in order to reduce the risks for the project sustainability, and that new sources of funding are needed for this purpose.     

Municipal solid waste composition and food loss reduction in Kyoto City

For 35 years, Kyoto City has conducted detailed household waste composition surveys under the guidance of Kyoto University by dividing household waste into approximately 400 categories. In addition, the city has conducted detailed composition surveys of commercial waste generated by businesses. These surveys show that food loss accounts for approximately 40% of total waste, of which leftovers and untouched food account for about 40% in both households and business facilities. Consequently, the annual generation of household and commercial food loss is estimated at about 30,000 tons. Various efforts have been made to reduce waste, including food loss, but further reduction in environmental burden is needed. Thus, Kyoto City revised the ordinance for waste reduction, and in March 2015, formulated a new municipal waste management plan. The plan not only includes the 2Rs (reduce, reuse), but also, for the first time in Japan, sets quantitative targets for reducing food loss. Kyoto City must ensure that the necessary waste reduction measures are clearly explained to the residents and business operators. To ensure that this plan is successful, it is important to clarify concrete actions that residents and business operators should implement, along with their effects.     

Quantitative analysis of perfluorinated chemicals in media for in vitro fertilization and related samples

The actual standard in vitro fertilization (IVF) protocol recommends an overnight gamete co-incubation. All of the culture media used for human IVF are supplemented with serum or albumin. In the present study, we determined the concentrations of perfluorinated chemicals (PFCs) in IVF media (IVFM) and related samples by liquid chromatography with tandem mass spectrometry (LC/MS/MS). The results indicated that the concentrations of PFOS and PFOA in the protein source were higher than those in the IVFM samples. Compared with human plasma concentrations of PFCs, PFCs in all of the IVFM samples, such as PFBS, PFHxS and PFOA, were either not detected or present at only trace levels, even when protein source was added. LC/MS/MS could be used to determine PFCs in IVFM samples in future studies of the effects of PFC exposure on intrauterine insemination.     

Effect of density-driven flow on the through-diffusion experiment

Diffusion is one of the main mechanisms of solute transport in pore water of geologic media. The effective diffusion coefficient of a solute in a rock is usually measured by the through-diffusion experiment. However, in this experiment, the effect of advection, induced by density difference between dense aqueous solution and pure water, has not been considered. To evaluate the effect of density-driven flow, a through-diffusion experiment using Fontainebleau sandstone was conducted for KCl and KI aqueous solutions with various densities. The measured effective diffusion coefficients were positively correlated with the density difference; the effective diffusion coefficient of a 1 M KI solution (density difference, 0.119 g/cm³) was one order of magnitude larger than that of a 0.1 M KCl solution (density difference, 0.005 g/cm³). The result is explained by a theoretical model using a diffusion–advection equation including Darcy's law. Based on the theory, a diagram to evaluate the condition at which the measured effective diffusion coefficient does not include the effect of advection is presented.     

Improvement of an efficient separation method for chemicals in diesel exhaust particles: analysis for nitrophenols

GOAL, SCOPE, AND BACKGROUND: Diesel exhaust is believed to consist of thousands of organic constituents and is a major cause of urban pollution. We recently reported that a systematic separation procedure involving successive solvent extractions, followed by repeated column chromatography, resulted in the isolation of vasodilatory active nitrophenols. These findings indicated that the estimation of the amount of nitrophenols in the environment is important to evaluate their effect on human health. The isolation procedure, however, involved successive solvent extractions followed by tedious, repeated chromatography, resulting in poor fractionation and in a significant loss of accuracy and reliability. Therefore, it was crucial to develop an alternative, efficient, and reliable analytical method. Here, we describe a facile and efficient acid-base extraction procedure for the analysis of nitrophenols. MATERIALS AND METHODS: Diesel exhaust particles (DEP) were collected from the exhaust of a 4JB1-type engine (ISUZU Automobile Co., Tokyo, Japan). Gas chromatography-mass spectrometry (GC-MS) analysis was performed with a GCMS-QP2010 instrument (Shimadzu, Kyoto, Japan). RESULTS: A solution of DEP in 1-butanol was extracted with aqueous NaOH to afford a nitrophenol-rich oily extract. The resulting oil was methylated with trimethylsilyldiazomethane and subsequently subjected to GC-MS analysis, revealing that 4-nitrophenol, 3-methyl-4-nitrophenol, 2-methyl-4-nitrophenol, and 4-nitro-3-phenylphenol were present in significantly higher concentrations than those reported previously. DISCUSSION: Simple acid-base extraction followed by the direct analysis of the resulting extract by GC-MS gave only broad peaks of nitrophenols with a poor detection limit, while the GC-MS analysis of the sample pretreated with (trimethylsilyl)diazomethane gave satisfactorily clear chromatograms with sharp peaks and with a significantly lowered detection limit (0.5 ng/ml, approximately 100 times). CONCLUSION: The present method involving an acid-base extraction, in situ derivatization, and GC-MS analysis has shown to be a simple, efficient, and reliable method for the isolation and identification of the chemical substances in DEP.     

Global fallout Pu recorded in lacustrine sediments in Lake Hongfeng, SW China

Studies on the distribution and isotope compositions of fallout Pu are important for source characterization of possible future non-fallout Pu contamination in aquatic environments, and useful for dating of recent sediments to understand the pollution history of environmental contaminants. We present the historical record of atmospheric Pu fallout reconstructed from a sediment core from Lake Hongfeng, China. The Pu activity profile was in agreement with the 137Cs profile. Inventories were 50.7 Bq m(-2) for 239+240Pu and 1586 Bq m(-2) for 137Cs. The average 240Pu/239Pu atom ratio was 0.185+/-0.009, indicating that Pu originated from global stratospheric fallout rather than from direct tropospheric or close-in fallout from the Chinese nuclear testing conducted in the 1970s. Our data suggested that Lake Hongfeng would be an ideal setting for monitoring atmospheric fallout and environmental changes in this region.     

绿色生产力:一个亚洲可持续发展的手段

绿色生产力的概念是由亚洲生产力组织(APO)提出的.绿色生产力是一种提高生产率和环境性能的战略.它的目的是要确保社会-经济的均衡发展,使得人类生活质量能够可持续性改善.在提高获利性和竞争优势的同时,运用适当的生产力与环境管理手段、技术和工艺,能够降低团体活动、产品和服务所造成的环境影响.利益相关者群体(包括政府、工业界和学术界)提出了改革建议,就是希望能够得到更大的可持续能力.     

PCDDs, PCDFs, and coplanar PCBs in wild terrestrial mammals from Japan: congener specific accumulation and hepatic sequestration

The present study determined the contamination levels and congener-specific accumulation features of dioxins and related compounds (DRCs) in wild terrestrial mammals such as large Japanese field mice (LJFM), lesser Japanese moles (LJMs), and raccoon dogs (RDs) collected from Kanto region in Japan during 2001. The toxic equivalent quantity (TEQ) levels in the carcasses or adipose tissues were in the order of RDs > or = LJMs > LJFM. Comparison of DRC congener profiles in the three species and principal component analysis (PCA) demonstrated a higher contribution of OCDD, T4CB77, and P5CB118 in LJMs. Analysis of liver-adipose distribution of DRC congeners in RDs showed that livers contained significantly higher TEQs than adipose tissues, indicating that liver is a depository organ and critical for determining the toxicokinetics of DRCs. As for most T4, P5, H6CDD/DFs and for P5CB126, H6CB169 and mono-ortho PCB congeners, their liver/adipose concentration ratios in RDs revealed a tendency to increase with hepatic TEQ levels, suggesting TEQ-dependent hepatic sequestration.